Proper patient selection is crucial to the success of prolotherapy regenerative treatments. Patients require a healthy healing potential and a strong immune system to benefit from this treatment. Good nutrition, exercise, and sleep habits are very important. Nutritional supplements including glucosamine, chondroitin sulfate, MSM, vitamin C, multivitamins/minerals are very beneficial. Poor prognosis is associated with patients who have primary discogenic pain or spine fusions. Individuals who are alcohol or drug abusers, heavy smokers, have chronic illnesses including diabetes and immunosuppressive diseases, have fibromyalgia usually do not respond as favorably.